Ultra Performance Liquid Chromatographic Technique for the Estimation of Brivaracetam and it’s Stress Degradation Studies

Abstract

Purpose: Sophisticated analytical chromatographic methods are important, convenient and relevant for the rapid and accurate determination of drugs and their related compounds in pharmaceutical dosage forms. Ultra performance liquid chromatography one of the fast and accurate liquid chromatographic method adopted in most of the advanced laboratories. The proposed work aims to develop a simple, rapid, sensitive, precise, accurate, economical and validated reverse phase ultra-performance liquid chromatographic (UPLC) method for the estimation of Brivaracetam in pure form and pharmaceutical dosage form.
Materials and Methods: The chromatographic separation was carried out on an Endoversil (50 x 2.1mm, 1.7µm) UPLC Column, a mixture of 0.1% OPA Buffer and Acetonitrile in the ratio of 30:70% v/v was used as a mobile phase. Flow rate of 0.25ml/min, 5μl injection volume, PDA detector with detection wavelength 265 nm was used for the assay. The retention time (RT) of Brivaracetam was found to be 0.523 ± 0.01 min.
Results and Discussion: Brivaracetam was linear with a correlation coefficient value 0.9999 in the concentration range of 6-14%. Specificity, accuracy (% mean recovery, 100.195%), precision, detection limits; robustness (% RSD˂2) and system suitability were found to be within limits. Degradation studies were performed under different stressed conditions, and the results of degradation studies reveal that the developed method was stable.
Conclusion: The developed method was simple, reliable, economical and stable and it can be applied for the routine quality control analysis of Brivaracetam in pure form and pharmaceutical dosage form.