Isolation, Characterization, and β-Cyclodextrin Nanosponge Formulation of Ochnaflavone from Ochna Obtusata for Enhanced Antioxidant Activity and Improved Drug Delivery

Abstract

Ochnaflavone, a biflavonoid isolated from the leaves of Ochna obtusata, was evaluated for its antioxidant activity and formulated into nanosponges to improve its solubility and bioavailability for potential Alzheimer's disease therapy. The compound was characterized using HPTLC, FTIR, and NMR techniques and demonstrated strong antioxidant activity in DPPH and H₂O₂ scavenging assays. Among the developed formulations, Trial 4 showed the best performance with high drug encapsulation efficiency (86.6%), a particle size of 193.2 nm, sustained drug release, and good stability. These findings suggest that Ochnaflavone-loaded nanosponges are a promising drug delivery system for future Alzheimer's disease treatment