Anti-Inflammatory Activity of Flavonoids Isolated from the Seed Extract of Glycine Max (Black) by In-Vitro and In-Vivo methods.

Abstract

Background: The Glycine max (L.) Merrill (black soybean) belongs to the family Fabaceae, from a high altitude geographic region is an abundant source of flavonoids. The flavonoids are well known for its diverse activities like anticancer, antidiabetic, anti-inflammatory, and hepatoprotective. The present study is carried out to investigate the anti-inflammatory efficacy of isolated flavonoids from seed extract of Glycine Max from Himalayan region (Uttarakhand, India) by in-vitro as well as in-vivo methods.
Methods: The anti-inflammatory activity of flavonoids isolated from 70 % hydroalcoholic seed extract was evaluated using a carrageenan (0.1 ml of 1% w/v carrageenan suspended in 1% carboxymethylcellulose) induced paw edema model in Wistar rats N=6 (Group I, II and III) having either sex with 170-200 gm body weight by in-vivo method. Group I served as control group and treated with distilled water orally, group II as test group treated with test extract dissolved in distilled water orally (250 mg/kg) and group III as standard group treated with Diclofenac sodium (10 mg/kg). Protein denaturation assay was used to screen the in-vitro anti-inflammatory activity of test extract.
Results: The paw edema was measured in volume (ml) at 0, 1st, 2nd, 3rd, 4th, and 5th hours after carrageenan injection by digital plethysmometer by in-vivo method. The obtained results were expressed in mean + SEM and statistical analysis was done by applying a one way ANOVA test followed by a post hoc Tukey test. The test group demonstrated significant anti-inflammatory efficacy as compared to the control and standard group by (p < 0.001) and (p < 0.05) respectively from in-vivo study. The protein denaturation assay showed highest inhibitory activity of Diclofenac sodium (80.37%) and the test extract (73.43%) at 100 µg/mL. The IC₅₀ values, calculated by linear interpolation, were 66.52 µg/mL for Diclofenac sodium and 69.92 µg/mL for the test extract.
Conclusions: The present study scientifically justifies flavonoids isolated from seed extract of Glycine Max have anti-inflammatory activity.