Renoprotective Potential of Boerhavia diffusa Root Extract in Cisplatin-Induced Nephrotoxicity

Abstract

Background: A standardized botanical extract could provide a mechanistically plausible adjunct for limiting platinum-associated renal injury. Objective: To evaluate standardized Boerhavia diffusa root extract in comparison with cisplatin injury control and healthy control using a controlled animal experiment. Methods: The draft protocol included 40 experimental units or participants involving adult male Wistar rats. The intervention was administered or tested for 10 days. The primary outcome was serum creatinine, with additional assessment of blood urea nitrogen, renal malondialdehyde, superoxide dismutase, tubular injury score. Standardized laboratory, clinical, or food-analysis procedures were applied, and between-condition effects were evaluated with two-sided tests at α=0.05. Results: Cisplatin increased serum creatinine to 2.31 ± 0.34 mg/dL; high-dose Boerhavia diffusa limited the increase to 0.96 ± 0.14 mg/dL (p<0.001 versus injury control).
The high-dose extract lowered renal malondialdehyde by 54.4%, restored superoxide dismutase activity to 34.1 ± 4.0 U/mg, and reduced the composite tubular injury score from 8.7 ± 1.0 to 2.2 ± 0.8. The direction and magnitude of the dummy findings were internally consistent across primary and secondary endpoints, and tolerability or assay-quality criteria remained acceptable. Conclusion: The sample data indicates that standardized Boerhavia diffusa root extract may lead to significant enhancement of serum creatinine with antioxidant preservation, lipid peroxidation suppression, and tubular inflammation. The findings are only to be taken as a complete manuscript-development model, and should be substituted with ethically created, self-confirmed findings prior to scientific presentation. Keywords: Boerhavia diffusa; cisplatin; nephrotoxicity; oxidative stress; renoprotection.