Molecular Detection and Antibiotic Resistance of Acinetobacter Bumannii Isolated from Mastitis Cases

Abstract

Mastitis in bovine remains as the great economic significant diseases disturbing lactating herds worldwide, with growing anxiety concerning infections caused by displaying resistance to several antibiotics. Acinetobacter baumannii has arisen as an opportunistic pathogen with valuable antimicrobial resistance characters, until now its role in bovine mastitis is still under-reported. The present study was conducted to investigate the isolate and identify A. baumannii from cases associated with both clinical and subclinical forms of mastitis in milk-producing cattle, to authorize the isolates at the level of molecular detection by real time PCR aiming the blaOXA-51 gene, and to assess their patterns of antimicrobial resistance. Overall 150 samples of milk were collected from cows with mastitis in several areas of Baghdad, Iraq. Standard bacteriological approaches, biochemical examinations, API 20E and RapID™ ONE system was used for phenotypic documentation. Molecular verification was carried out utilizing real time PCR. Antibiotics susceptibility analysis exposed high rates of reduce susceptibility to numerous aminoglycosides and β-lactams, while variable susceptibility was detected tocolistin, carbapenems, and tetracycline derivatives. The recognition of the intrinsic blaOXA-51 gene established the identity the isolates of A. baumannii. The rate of contamination of A. baumannii in mastitis cases reach 55 of 150 samples (36.67%). These results highlight the possible role of A. baumannii as mastitis related pathogen and underline the necessity for unceasing surveillance of resistance to antimicrobials in dairy farms.