Hormones with Sperm and Testes in Albino Male Mice Effected by Treated with Phthalate Migration from Bottled Water

Abstract

Phthalates, such as including type dibutyl phthalate (DBP), or phthalic acid esters, chemical are widely used in industry as plasticizers for many polymer products and are present in bottled water and. Effects of male infertility and testes after detected in bottled water. In this study, we aimed to examine the reproductive toxicity resulting from simultaneous exposure dibutyl phthalate (DBP) in male albino mice. The toxicity was evaluated by causing adverse effects on the male reproductive system, The mice were divided into four groups, and each group was  treated for (DBP) at three concentrations (3-7-15) mg/kg for 45 days, except control group, its effect on FSH hormones in highest concentration was recorded (17.79 mIU/ml), in (2.06 mIU/ml) for LH and testosterone (1.85 ng/ml) ,when Changes in sperm count from (31.67) (sperm x106) to decrease in control group(19.67) (sperm x106) at15 mg/kg were observed, along with a decrease in motility and turbidity, and an increase in mortality (23.67%) compared to the control group. It also affected testicular tissue by affecting the appearance of the seminiferous tubules, with swelling in the tissue due to exposure to phthalates at concentrations of (7 -15) mg/kg respectively. Finaly, examination of the comet revealed a toxic relationship to the DNA strand, which was at its shortest in length at the first concentration and longest comet at the second and third concentrations as a result of chronic exposure to the specified doses.