Association of Antimicrobial Peptides (HNP1-3 And HBD3) and Adaptive Immunity Cytokines (IL10 And INF-Γ) with Disease Extent, Bacterial Burdens and Severity in Patients With Pulmonary Tuberculosis.

Abstract

limited information regarding the balance between cytokines secreted from Th1/Th2 cells and their correlation with AMP before and during treatment of tuberculosis. This study aimed to investigate levels of cytokines (IL10 and INF-γ) and certain antimicrobial peptides (HNP1-3 and HBD3) in pulmonary tuberculosis patients (active and treated) and their correlation with the disease severity and sputum smear grade.
Sixty patients with PTB (Classified as 30 with Active PTB and 30 undertreatment PTB patient) compared to 30 apparently healthy subjects were enrolled in this case-control study. Blood samples were collected from each patient. Circulating levels of INF γ, IL10, HNP1–3 and HBD3 was measured using ELISA technique.
Significant higher concentration of HNP 1-3 in both patient groups in comparison to healthy, whereas, non-significant lower mean of HBD3 was observed in active patients. Decrease IL 10 with increase severity, higher mean of INF-γ and HBD 3 were found in moderate severity cases, whereas, HNP1-3 tend to increase from mild to severe cases. Lower mean levels IL10 and HBD3 mean was found in +3 grade., whereas, lowest mean of INF-γ and HNP1-3 was seen in +2 grade. ROC analysis showed that HNP 1-3 had higher discriminatory power indiscrimination. This study might possibly reflect the importance of the tested biomarkers in pathogenesis of TB and bacterial clearance.