Correlation Between Histidine-Rich Glycoprotein and Thrombospondin-1: Potential Role in Angiogenic Regulation in Idiopathic Recurrent Miscarriage

Abstract

Background: Recurrent miscarriage (RM) is defined by the European Society of Human Reproduction and Embryology (ESHRE) as the loss of two or more pregnancies, irrespective of their sequence. several studies have associated angiogenesis with recurrent miscarriage. Moreover, various factors have been identified as participating in the angiogenesis process, including histidine-rich glycoprotein (HRG) and thrombospondin-1 (TSP-1). Objective: The present research set out to investigate the angiogenic function of correlation between TSP-1 and HRG in order to find out its relevance in the pathophysiology of idiopathic recurrent miscarriage.
Material and Methods: A case-control research was performed with 70 women at idiopathic RM and 70 pregnant controls who had at least one live birth. Participants both patients and controls were matched in ages (18-35) years, their husbands ages (20-40) years, and BMI ranged (18.5-29.9) kg/m². Blood samples (10 ml) were obtained from participants. Laboratory testing were employed to exclude probable causes of RM by confirming that they were within normal limits. Stored serum at -80◦c were used to examine HRG and TSP-1 concentrations by ELISA technique.
Results: Women with idiopathic recurrent miscarriages had significantly lower levels of histidine rich glycoprotein (P <0.001), whereas higher levels of thrombospondine-1 (P=0.00071) than controls. Furthermore, invers correlation between these two parameters were noted among patient group. Conclusion: The reduction in HRG, along with the elevation of TSP-1 and the inverse correlation between these biomarkers in women undergoing idiopathic recurrent miscarriage, may result in a dysregulation of angiogenesis, perhaps associated with idiopathic recurrent miscarriages.